| 质粒名称: | pMJ326: pCMV-GtFz1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 205264 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 完整质粒大小: | 5786bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GtFz1 |
| 物种来源: | Guillardia theta |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | pMJ326: pCMV-GtFz1 was a gift from Feng Zhang (Addgene plasmid # 205264 ; http://n2t.net/addgene:205264 ; RRID:Addgene_205264) |
| 参考文献部分: | Fanzor is a eukaryotic programmable RNA-guided endonuclease. Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S, Desimone A, Macrae RK, Zhang F. Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2. 10.1038/s41586-023-06356-2 PubMed 37380027 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMJ326: pCMV-GtFz1 5786 bp ds-DNA circular SYN 15-AUG-2023
DEFINITION Human expression vector for human codon optimized GtFz1.
ACCESSION .
VERSION .
KEYWORDS pMJ326: pCMV-GtFz1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5786)
AUTHORS Saito M, Xu P, Faure G, Maguire S, Kannan S, Altae-Tran H, Vo S,
Desimone A, Macrae RK, Zhang F
TITLE Fanzor is a eukaryotic programmable RNA-guided endonuclease.
JOURNAL Nature. 2023 Jun 28. doi: 10.1038/s41586-023-06356-2.
PUBMED 37380027
REFERENCE 2 (bases 1 to 5786)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 15, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5786
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(2732..2751)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 2797..2852
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(2851..2870)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3213..3229)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3213..3229)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3226..3248)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3237..3253
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3261..3291)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3306..3327
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3386..3581
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 3432..3567
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3494..3513
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 3587..3721
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3624..3643)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3678..3697
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3789..3806)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3960..4548)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4040..4059)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4719..5579)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5342..5361
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5580..5684)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggactcag atcgccacca tgagcaacat ccggatcgtg aagcggaagg ccaagggctt
601 cttcaagtgc gaggacctgg tcaccatcaa ggacgccgtg aaagccgctc accggatcat
661 gtctgatgcc agcattctcg tgcggagcta ctacctgaga tggttccaga gcagctaccc
721 tctggacagc gacgacaaag agctggaact ggaacacttc cacatcagca tggcctgcag
781 catcgtgcag ggcatcacaa gacctccagt tagaggcgtg ggccctgagc agagcgtgaa
841 gatcgacgtg ttcaacgaca tgctggacga gtacaagcgg ctgtacgaga gagcccctaa
901 cgacaaagaa aacgagacag acctgagcct gagccacgtg ctggcctact ccatcgacaa
961 tctgctgacc gcctacaaga acaacatcga ggcccacttc agcaaatacg tcaagcggtt
1021 catccgctgc gatatgctgg ccaagggatt caacaagtct gaggccaaca gagtggccgc
1081 catctacacc aacgcctaca tctacgacag cagcctggac ctggaacctg acttcatgga
1141 acggctggga ctcgaggcca ccagctactc tagcctgttt cctagcaaga tcaacaaagg
1201 cggcttcccc agagtgtacg acctgaaggc taacccctgg gtgtacctgc ctaagatggt
1261 catgatcaat caggccctgg aaaccgactt ctccagcgtg gaacacaaag agcggcggct
1321 gctgaaccct ctgcctttct acagcagctt cgtgcccatg cacatcagaa tcgacaccag
1381 cggcctgtct cagctgctga tgaccaagga cagactggac gacttcaaga gaagctacct
1441 ggccgagttc ggcgtgtccc tgaacatcaa gaacaaaggg gacatgctcg ccagcttcga
1501 gaagatcttt ggcagaaagg ccacctccaa tagagaggcc ggactgtacg ctaccgagat
1561 gtggtccttc ctgaccaacc tgaaaacctg caggcagtgg aaagaactgg acggcgtcgt
1621 gcggaagaac gaccctaagg gaacccagtg gatgttcgac aacgccgtgg tcacagacgg
1681 cgtcagcatc agcttccaag tgatcgacaa cagcatgttc ggccgcaagg ccttcagcgg
1741 ccggaagaaa agagtggcct gtcaagaggc caacgacgaa gaggacagca agcaagtgac
1801 ccgcgaggaa ctgaaaacca gcaagctgct gggctgcgac cccggcaaga gagatatcct
1861 ggccatcacc gacggcatca agaccatctg ctacaccaag ggccagagag acatggacac
1921 ccacaagaca atccggctgc ggaccagcct gaagagaaga agaggttgcg gcctggaaga
1981 gtacgagaca caagtgatga atcggttcca gaagcggagc tgtcaccccg agatgttcag
2041 aagatacgcc tgcagccgga agcggatgga acacatgctg ctggaatgct actctcaccc
2101 cgtgttccgc gagttcaagt tcctggtgta caacaagacc aagagcagcg agcaccggtt
2161 catgcacaga gtgctggaaa ccttcaagcg gccccagacc aatctgagca aggccagatg
2221 tgccagcggc gtgatgagaa tgaacgccct gaaagaggtg cagcggcacg gcgatatcat
2281 catcggctgg ggcaattggg gcaagaaccc caacgctctg agatgctctg ccggacctac
2341 acctggcatc ggcatcagaa gaagattcga gagcctgttc aagaccacca ccgtgcctga
2401 gcactacacc agccaagagt gccctagctg caagggcaga tgcctgagaa aagccaccgg
2461 caatcccatc atgcggcatc atctgctgcg gtgcaccaac gatagctgct gctccagatg
2521 gtggaaccgg aatgtggctg gcgccttcaa catcctgacc agactgctgg atggccagac
2581 actgagcggc aatgagacaa caggcgacgg actcggcgga gatgatctgt gactagcata
2641 accccttggg gcgcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
2701 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
2761 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
2821 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
2881 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
2941 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
3001 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
3061 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
3121 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
3181 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
3241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
3301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
3361 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
3421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
3481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
3541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
3601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
3661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
3721 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
3781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
3841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
3901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
3961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4381 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
4441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
4621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
4681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
4741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
4801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
4861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
4921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
4981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
5341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
5581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
5641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
5701 gtgccacctg ggtcgacatt gattattgac tagtaatagt aatcaattac ggggtcatta
5761 gttcatagcc catatatgga gttccg
//
